IL-4 anticorps (Biotin)

N° du produit ABIN115955

L’anticorps Lapin Polyclonal anti-IL-4 a été validé pour WB et EIA. Il convient pour détecter IL-4 dans des échantillons de Rat.

Aperçu rapide pour IL-4 anticorps (Biotin) (ABIN115955)

Antigène: IL-4 (IL4) (Interleukin 4 (IL4))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IL-4 est conjugé à/à la Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-IL-4 anticorps (Biotin)

Specificité: This antibody recognises Rat IL-4.

Purification: Affinity chromatography.

Immunogène: Highly pure (>98%) recombinant rat IL-4 (rat Interleukin-4).

Information d'application

Indications d'application: ELISA: To detect rat IL-4 by direct ELISA (using 100 ml/well antibody solution) this antibody can beused at a concentration of 0.15-0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant rat IL-4. Western Blot: To detect rat IL-4 by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant rat IL-4 is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Détails sur IL-4

Antigène: IL-4 (IL4) (Interleukin 4 (IL4))

Autre désignation: Interleukin-4 / IL4

Sujet: IL4 is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for Interleukin 4 receptor. The Interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naive CD4+ T cell to the Th2 type. Th2 cells produce IL4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.Synonyms: B-cell stimulatory factor 1, BSF1, Binetrakin, IL-4, Lymphocyte stimulatory factor 1, Pitrakinra

ID gène: 287287

NCBI Accession: NP_958427

UniProt: P20096

Pathways: Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation